› 4n0l

X-ray diffraction
2.37Å resolution

Methanopyrus kandleri Csm3 crystal structure

Released:
DOI: 10.2210/pdb4n0l/pdb
PDB entry 4n0l coloured by chain, front view.
PDB entry 4n0l coloured by chain, side view.
PDB entry 4n0l coloured by chain, top view.
Source organism: Methanopyrus kandleri AV19
Primary publication:
Structure and RNA-binding properties of the type III-A CRISPR-associated protein Csm3.
Hrle A, Su AA, Ebert J, Benda C, Randau L, Conti E
RNA Biol 10 1670-8 (2013)
PMID: 24157656

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186302 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR system Cms endoribonuclease Csm3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 39.62 KDa
Source organism: Methanopyrus kandleri AV19
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TVS2 (Residues: 1-351; Coverage: 100%)
Gene name: MK1316
Sequence domains: RAMP superfamily

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 95.753Å b: 101.017Å c: 174.167Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.216
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Unravelling the structural and mechanistic basis of CRISPR-Cas systems.
van der Oost et al. (2014)
5 more

6 mentions without citation

Annotation and Classification of CRISPR-Cas Systems.
Makarova et al. (2015)
5 more