› 4n0q

X-ray diffraction
2.3Å resolution

Crystal Structure of an ABC transporter, substrate-binding protein from Brucella melitensis 16M in complex with L-Leucine using a crystal grown in a Crystal Former (Microlytic)

Released:
DOI: 10.2210/pdb4n0q/pdb
PDB entry 4n0q coloured by chain, front view.
PDB entry 4n0q coloured by chain, side view.
PDB entry 4n0q coloured by chain, top view.
Source organism: Brucella melitensis bv. 1 str. 16M
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187043 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leu/Ile/Val-binding protein homolog 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 354 amino acids
Theoretical weight: 36.73 KDa
Source organism: Brucella melitensis bv. 1 str. 16M
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YEE8 (Residues: 23-368; Coverage: 100%)
Gene name: BMEI1930
Sequence domains: Periplasmic binding protein
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand LEU 4 x LEU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 69.5Å b: 59.85Å c: 147.9Å
α: 90° β: 91.22° γ: 90°
R-values:
R R work R free
0.215 0.212 0.269
Expression system: Escherichia coli

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