› 4n0t

X-ray diffraction
1.7Å resolution

Core structure of the U6 small nuclear ribonucleoprotein at 1.7 Angstrom resolution

Released:
DOI: 10.2210/pdb4n0t/pdb
PDB entry 4n0t coloured by chain, front view.
PDB entry 4n0t coloured by chain, side view.
PDB entry 4n0t coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Core structure of the U6 small nuclear ribonucleoprotein at 1.7-Å resolution.
Montemayor EJ, Curran EC, Liao HH, Andrews KL, Treba CN, Butcher SE, Brow DA
Nat Struct Mol Biol 21 544-51 (2014)
PMID: 24837192

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156106 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U4/U6 snRNA-associated-splicing factor PRP24 Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 43.25 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P49960 (Residues: 34-400; Coverage: 83%)
Gene names: PRP24, YM8156.10C, YMR268C
Sequence domains:
Structure domains: Alpha-Beta Plaits
U6 snRNA Chain: B
Molecule details ›
Chain: B
Length: 72 nucleotides
Theoretical weight: 23.18 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
Sequence domains: U6 spliceosomal RNA

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 61.782Å b: 71.37Å c: 82.081Å
α: 90° β: 109.62° γ: 90°
R-values:
R R work R free
0.184 0.184 0.211
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

14 review citations

Mechanisms and Regulation of Alternative Pre-mRNA Splicing.
Lee et al. (2015)
13 more

8 mentions without citation

Ribosome-dependent activation of stringent control.
Brown et al. (2016)
7 more