› 4n1j

X-ray diffraction
2.6Å resolution

Crystal structures of NLRP14 pyrin domain reveal a conformational switch mechanism, regulating its molecular interactions

Released:
DOI: 10.2210/pdb4n1j/pdb
PDB entry 4n1j coloured by chain, front view.
PDB entry 4n1j coloured by chain, side view.
PDB entry 4n1j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the NLRP14 pyrin domain reveal a conformational switch mechanism regulating its molecular interactions.
Eibl C, Hessenberger M, Wenger J, Brandstetter H
Acta Crystallogr D Biol Crystallogr 70 2007-18 (2014)
PMID: 25004977

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-183221 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NACHT, LRR and PYD domains-containing protein 14 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 105 amino acids
Theoretical weight: 12.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86W24 (Residues: 1-100; Coverage: 9%)
Gene names: NALP14, NLRP14, NOD5
Sequence domains: PAAD/DAPIN/Pyrin domain
Structure domains: Death Domain, Fas

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P63
Unit cell:
a: 89.61Å b: 89.61Å c: 107.871Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.209 0.255
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

International Union of Basic and Clinical Pharmacology. XCVI. Pattern recognition receptors in health and disease.
Bryant et al. (2015)
2 more

5 mentions without citation

Structural mechanisms in NLR inflammasome signaling.
Lechtenberg et al. (2014)
4 more