› 4n2s

X-ray diffraction
3Å resolution

Crystal Structure of THA8 in complex with Zm1a-6 RNA

Released:
DOI: 10.2210/pdb4n2s/pdb
PDB entry 4n2s coloured by chain, front view.
PDB entry 4n2s coloured by chain, side view.
PDB entry 4n2s coloured by chain, top view.
Source organism: Brachypodium distachyon
Primary publication:
Structural basis for RNA recognition by a dimeric PPR-protein complex.
Ke J, Chen RZ, Ban T, Zhou XE, Gu X, Tan MH, Chen C, Kang Y, Brunzelle JS, Zhu JK, Melcher K, Xu HE
Nat Struct Mol Biol 20 1377-82 (2013)
PMID: 24186060

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125011 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Pentacotripeptide-repeat region of PRORP domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 27.75 KDa
Source organism: Brachypodium distachyon
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: I1HB13 (Residues: 1-257; Coverage: 100%)
Gene names: 100823749, BRADI_2g00245v3, BRADI_2g00270v3
Structure domains: Tetratricopeptide repeat domain
Zm1a-6 RNA Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.84 KDa
Source organism: Brachypodium distachyon
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P41212
Unit cell:
a: 88.25Å b: 88.25Å c: 78.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.232
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

19 review citations

Pentatricopeptide repeat proteins in plants.
Barkan et al. (2014)
18 more