› 4n49

X-ray diffraction
1.9Å resolution

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with m7GpppG and SAM

Released:
DOI: 10.2210/pdb4n49/pdb
PDB entry 4n49 coloured by chain, front view.
PDB entry 4n49 coloured by chain, side view.
PDB entry 4n49 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of human 2'-O-ribose methyltransferases involved in mRNA cap structure formation.
Smietanski M, Werner M, Purta E, Kaminska KH, Stepinski J, Darzynkiewicz E, Nowotny M, Bujnicki JM
Nat Commun 5 3004 (2014)
PMID: 24402442

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185357 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 48.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N1G2 (Residues: 126-550; Coverage: 51%)
Gene names: CMTR1, FTSJD2, KIAA0082, MTR1
Sequence domains: FtsJ-like methyltransferase
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
2 bound ligands:
Ligand MGT 1 x MGT
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 50.834Å b: 87.619Å c: 57.31Å
α: 90° β: 112.89° γ: 90°
R-values:
R R work R free
0.156 0.154 0.203
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Innate immune restriction and antagonism of viral RNA lacking 2׳-O methylation.
Hyde et al. (2015)
15 more

13 mentions without citation

RNA methyltransferases involved in 5' cap biosynthesis.
Byszewska et al. (2014)
12 more