› 4n53

X-ray diffraction
3.31Å resolution

Human enterovirus 71 uncoating intermediate captured at atomic resolution

Released:
DOI: 10.2210/pdb4n53/pdb
PDB entry 4n53 coloured by chain, front view.
PDB entry 4n53 coloured by chain, side view.
PDB entry 4n53 coloured by chain, top view.
Source organism: Enterovirus A71
Primary publication:
Human enterovirus 71 uncoating captured at atomic resolution.
Lyu K, Ding J, Han JF, Zhang Y, Wu XY, He YL, Qin CF, Chen R
J Virol 88 3114-26 (2014)
PMID: 24352461

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
PDBe Complex ID:
PDB-CPX-197423 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Capsid protein VP1 Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 32.7 KDa
Source organism: Enterovirus A71
UniProt:
  • Canonical: S5QA87 (Residues: 1-297; Coverage: 100%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP2 Chain: B
Molecule details ›
Chain: B
Length: 254 amino acids
Theoretical weight: 27.73 KDa
Source organism: Enterovirus A71
UniProt:
  • Canonical: S4VM80 (Residues: 70-323; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP3 Chain: C
Molecule details ›
Chain: C
Length: 242 amino acids
Theoretical weight: 26.44 KDa
Source organism: Enterovirus A71
UniProt:
  • Canonical: S5ZCI0 (Residues: 324-565; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP4 Chain: D
Molecule details ›
Chain: D
Length: 69 amino acids
Theoretical weight: 7.5 KDa
Source organism: Enterovirus A71
UniProt:
  • Canonical: S6C3M9 (Residues: 1-69; Coverage: 47%)
Sequence domains: Picornavirus coat protein (VP4)
Structure domains: Rhinovirus 14, subunit 4

Ligands and Environments

1 bound ligand:
Ligand SPH 1 x SPH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: I23
Unit cell:
a: 591.375Å b: 591.375Å c: 591.375Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.261

Quick links

Citations

5 review citations

Enterovirus A71 Proteins: Structure and Function.
Yuan et al. (2018)
4 more

4 mentions without citation

Human enterovirus 71 uncoating captured at atomic resolution.
Lyu et al. (2014)
3 more