› 4n69

X-ray diffraction
1.8Å resolution

Soybean Serine Acetyltransferase Complexed with Serine

Released:
DOI: 10.2210/pdb4n69/pdb
PDB entry 4n69 coloured by chain, front view.
PDB entry 4n69 coloured by chain, side view.
PDB entry 4n69 coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Structure of soybean serine acetyltransferase and formation of the cysteine regulatory complex as a molecular chaperone.
Yi H, Dey S, Kumaran S, Lee SG, Krishnan HB, Jez JM
J Biol Chem 288 36463-72 (2013)
PMID: 24225955

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
serine O-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 30.4 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: I1KHY6 (Residues: 1-286; Coverage: 100%)
Gene names: 100815091, GLYMA_07G058800
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SER 2 x SER
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 111.368Å b: 111.368Å c: 143.456Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.167 0.196
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Moonlighting O-acetylserine sulfhydrylase: New functions for an old protein.
Campanini et al. (2015)
3 more

3 mentions without citation

New insights into the structure and function of an emerging drug target CysE.
Verma et al. (2021)
2 more