› 4n6l

X-ray diffraction
1.95Å resolution

Crystal structure of human cystatin E/M

Released:
DOI: 10.2210/pdb4n6l/pdb
PDB entry 4n6l coloured by chain, front view.
PDB entry 4n6l coloured by chain, side view.
PDB entry 4n6l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and mechanism of an aspartimide-dependent peptide ligase in human legumain.
Dall E, Fegg JC, Briza P, Brandstetter H
Angew Chem Int Ed Engl 54 2917-21 (2015)
PMID: 25630877

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystatin-M Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15828 (Residues: 29-149; Coverage: 100%)
Gene name: CST6
Sequence domains: Cystatin domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 42.73Å b: 68.6Å c: 40.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.24
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Macrocyclization by asparaginyl endopeptidases.
James et al. (2018)
8 more

3 mentions without citation

High Molecular Weight Kininogen: A Review of the Structural Literature.
Ponczek MB. (2021)
2 more