› 4n6w

X-ray diffraction
1.85Å resolution

X-Ray Crystal Structure of Citrate-bound PhnZ

Released:
DOI: 10.2210/pdb4n6w/pdb
PDB entry 4n6w coloured by chain, front view.
PDB entry 4n6w coloured by chain, side view.
PDB entry 4n6w coloured by chain, top view.
Source organism: uncultured bacterium HF130_AEPn_1
Primary publication:
Organophosphonate-degrading PhnZ reveals an emerging family of HD domain mixed-valent diiron oxygenases.
Wörsdörfer B, Lingaraju M, Yennawar NH, Boal AK, Krebs C, Bollinger JM, Pandelia ME
Proc Natl Acad Sci U S A 110 18874-9 (2013)
PMID: 24198335

Function and Biology Details

Reaction catalysed: 
(2-amino-1-hydroxyethyl)phosphonate + O(2) = glycine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-amino-1-hydroxyethylphosphonate dioxygenase (glycine-forming) Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 26.07 KDa
Source organism: uncultured bacterium HF130_AEPn_1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0E8I5 (Residues: 1-190; Coverage: 100%)
Gene names: ALOHA_HF130_AEPn_1_06c, phnZ
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

2 bound ligands:
Ligand FE 2 x FE
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P3221
Unit cell:
a: 68.871Å b: 68.871Å c: 98.842Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Dioxygen Activation by Nonheme Diiron Enzymes: Diverse Dioxygen Adducts, High-Valent Intermediates, and Related Model Complexes.
Jasniewski et al. (2018)
3 more

3 mentions without citation

Organophosphonate-degrading PhnZ reveals an emerging family of HD domain mixed-valent diiron oxygenases.
Wörsdörfer et al. (2013)
2 more