› 4n75

X-ray diffraction
2.6Å resolution

Structural Basis of BamA-mediate Outer Membrane Protein Biogenesis

Released:
DOI: 10.2210/pdb4n75/pdb
PDB entry 4n75 coloured by chain, front view.
PDB entry 4n75 coloured by chain, side view.
PDB entry 4n75 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural and functional analysis of the β-barrel domain of BamA from Escherichia coli.
Ni D, Wang Y, Yang X, Zhou H, Hou X, Cao B, Lu Z, Zhao X, Yang K, Huang Y
FASEB J 28 2677-85 (2014)
PMID: 24619089

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein assembly factor BamA Chains: A, B
Molecule details ›
Chains: A, B
Length: 385 amino acids
Theoretical weight: 43.04 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A940 (Residues: 427-810; Coverage: 49%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains: Omp85 superfamily domain
Structure domains: membrane protein fhac: a member of the omp85/tpsb transporter family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 118.492Å b: 159.883Å c: 56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.205 0.253
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Emerging Diversity in Lipid-Protein Interactions.
Corradi et al. (2019)
13 more

23 mentions without citation

The β-barrel assembly machinery in motion.
Noinaj et al. (2017)
22 more