› 4n7w

X-ray diffraction
1.95Å resolution

Crystal Structure of the sodium bile acid symporter from Yersinia frederiksenii

Released:
DOI: 10.2210/pdb4n7w/pdb
PDB entry 4n7w coloured by chain, front view.
PDB entry 4n7w coloured by chain, side view.
PDB entry 4n7w coloured by chain, top view.
Source organism: Yersinia frederiksenii
Primary publication:
Structural basis of the alternating-access mechanism in a bile acid transporter.
Zhou X, Levin EJ, Pan Y, McCoy JG, Sharma R, Kloss B, Bruni R, Quick M, Zhou M
Nature 505 569-73 (2014)
PMID: 24317697

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-164864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transporter, sodium/bile acid symporter family Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 32.72 KDa
Source organism: Yersinia frederiksenii
Expression system: Escherichia coli BL21(DE3)
Structure domains: Na+/H+ antiporter like fold

Ligands and Environments

2 bound ligands:
Ligand CIT 2 x CIT
Ligand MPG 2 x MPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 189.406Å b: 46.223Å c: 70.357Å
α: 90° β: 100.68° γ: 90°
R-values:
R R work R free
0.188 0.186 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

22 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
21 more

12 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
11 more