› 4nas

X-ray diffraction
1.92Å resolution

The crystal structure of a rubisco-like protein (MtnW) from Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Released:
DOI: 10.2210/pdb4nas/pdb
PDB entry 4nas coloured by chain, front view.
PDB entry 4nas coloured by chain, side view.
PDB entry 4nas coloured by chain, top view.
Source organism: Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Entry authors: Tan K, Li H, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribulose-bisphosphate carboxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 409 amino acids
Theoretical weight: 44.38 KDa
Source organism: Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: C8WQ56 (Residues: 3-408; Coverage: 99%)
Gene name: Aaci_0096
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GOL 1 x GOL
Ligand FMT 3 x FMT
Ligand CA 7 x CA
Ligand CL 1 x CL
2 modified residues:
Ligand MSE 24 x MSE
Ligand KCX 4 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 56.763Å b: 175.671Å c: 83.653Å
α: 90° β: 107.67° γ: 90°
R-values:
R R work R free
0.198 0.195 0.251
Expression system: Escherichia coli BL21

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