› 4nef

X-ray diffraction
2.75Å resolution

X-ray structure of human Aquaporin 2

Released:
DOI: 10.2210/pdb4nef/pdb
PDB entry 4nef coloured by chain, front view.
PDB entry 4nef coloured by chain, side view.
PDB entry 4nef coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray structure of human aquaporin 2 and its implications for nephrogenic diabetes insipidus and trafficking.
Frick A, Eriksson UK, de Mattia F, Oberg F, Hedfalk K, Neutze R, de Grip WJ, Deen PM, Törnroth-Horsefield S
Proc Natl Acad Sci U S A 111 6305-10 (2014)
PMID: 24733887

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aquaporin-2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 242 amino acids
Theoretical weight: 25.24 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: P41181 (Residues: 3-241; Coverage: 88%)
Gene name: AQP2
Sequence domains: Major intrinsic protein
Structure domains: Glycerol uptake facilitator protein.

Ligands and Environments

2 bound ligands:
Ligand CD 2 x CD
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P42
Unit cell:
a: 119.11Å b: 119.11Å c: 90.621Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.225
Expression system: Komagataella phaffii GS115

Quick links

Citations

12 review citations

Pathophysiology, diagnosis and management of nephrogenic diabetes insipidus.
Bockenhauer et al. (2015)
11 more

28 mentions without citation

Aquaporin Protein-Protein Interactions.
Roche et al. (2017)
27 more