› 4nel

X-ray diffraction
2.05Å resolution

Crystal structure of a putative transcriptional regulator from Saccharomonospora viridis in complex with N,N-dimethylmethanamine

Released:
DOI: 10.2210/pdb4nel/pdb
PDB entry 4nel coloured by chain, front view.
PDB entry 4nel coloured by chain, side view.
PDB entry 4nel coloured by chain, top view.
Source organism: Saccharomonospora viridis DSM 43017
Entry authors: Halavaty AS, Filippova EV, Minasov G, Kiryukhina O, Shuvalova L, Endres M, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111722 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 22.34 KDa
Source organism: Saccharomonospora viridis DSM 43017
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C7MT25 (Residues: 95-284; Coverage: 66%)
Gene name: Svir_16270
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

1 bound ligand:
Ligand KEN 1 x KEN
2 modified residues:
Ligand MSE 2 x MSE
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 41.864Å b: 109.24Å c: 73.878Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.259
Expression system: Escherichia coli BL21(DE3)

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