› 4neo

X-ray diffraction
2.1Å resolution

Structure of BlmI, a type-II acyl-carrier-protein from Streptomyces verticillus involved in bleomycin biosynthesis

Released:
DOI: 10.2210/pdb4neo/pdb
PDB entry 4neo coloured by chain, front view.
PDB entry 4neo coloured by chain, side view.
PDB entry 4neo coloured by chain, top view.
Source organism: Streptomyces verticillus
Primary publication:
The crystal structure of BlmI as a model for nonribosomal peptide synthetase peptidyl carrier proteins.
Lohman JR, Ma M, Cuff ME, Bigelow L, Bearden J, Babnigg G, Joachimiak A, Phillips GN, Shen B
Proteins 82 1210-8 (2014)
PMID: 25050442

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carrier domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 10.37 KDa
Source organism: Streptomyces verticillus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9XC48 (Residues: 2-90; Coverage: 99%)
Gene name: blmI
Sequence domains: Phosphopantetheine attachment site
Structure domains: ACP-like

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand PG0 1 x PG0
2 modified residues:
Ligand MSE 2 x MSE
Ligand MLY 1 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3221
Unit cell:
a: 73.441Å b: 73.441Å c: 43.331Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.18 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The structural biology of biosynthetic megaenzymes.
Weissman KJ. (2015)
4 more

4 mentions without citation

Data to knowledge: how to get meaning from your result.
Berman et al. (2015)
3 more