› 4nfp

X-ray diffraction
1.85Å resolution

Crystal Structure Analysis of the 16mer GCAGNCUUAAGUCUGC containing 8-aza-7-deaza-7-ethynyl Adenosine

Released:
DOI: 10.2210/pdb4nfp/pdb
PDB entry 4nfp coloured by chain, front view.
PDB entry 4nfp coloured by chain, side view.
PDB entry 4nfp coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Click modification of RNA at adenosine: structure and reactivity of 7-ethynyl- and 7-triazolyl-8-aza-7-deazaadenosine in RNA.
Phelps KJ, Ibarra-Soza JM, Tran K, Fisher AJ, Beal PA
ACS Chem Biol 9 1780-7 (2014)
PMID: 24896732

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196840 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
GCAG(A7E)CUUAAGUCUGC Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 16 nucleotides
Theoretical weight: 5.12 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ARF 3 x ARF
1 modified residue:
Ligand A7E 3 x A7E

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 74.8Å b: 43.19Å c: 48.79Å
α: 90° β: 120.82° γ: 90°
R-values:
R R work R free
0.224 0.222 0.249
Expression system: Not provided

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Citations

3 review citations

Therapeutic siRNA: state of the art.
Hu et al. (2020)
2 more