› 4nh0

X-ray diffraction
2.9Å resolution

Cytoplasmic domain of the Thermomonospora curvata Type VII Secretion ATPase EccC

Released:
DOI: 10.2210/pdb4nh0/pdb
PDB entry 4nh0 coloured by chain, front view.
PDB entry 4nh0 coloured by chain, side view.
PDB entry 4nh0 coloured by chain, top view.
Source organism: Thermomonospora curvata DSM 43183
Primary publication:
Substrates Control Multimerization and Activation of the Multi-Domain ATPase Motor of Type VII Secretion.
Rosenberg OS, Dovala D, Li X, Connolly L, Bendebury A, Finer-Moore J, Holton J, Cheng Y, Stroud RM, Cox JS
Cell 161 501-512 (2015)
PMID: 25865481

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ESX secretion system protein EccC Chains: A, B
Molecule details ›
Chains: A, B
Length: 1147 amino acids
Theoretical weight: 126.47 KDa
Source organism: Thermomonospora curvata DSM 43183
Expression system: Escherichia coli
UniProt:
  • Canonical: D1A4G7 (Residues: 200-1315; Coverage: 85%)
Gene names: Tcur_0607, eccC
Sequence domains: FtsK/SpoIIIE family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand MG 4 x MG
Ligand ATP 4 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 251.816Å b: 116.316Å c: 174.11Å
α: 90° β: 102.7° γ: 90°
R-values:
R R work R free
0.226 0.226 0.246
Expression system: Escherichia coli

Quick links

Citations

15 review citations

ESX secretion systems: mycobacterial evolution to counter host immunity.
Gröschel et al. (2016)
14 more

4 mentions without citation

The structure of the endogenous ESX-3 secretion system.
Poweleit et al. (2019)
3 more