› 4nhj

X-ray diffraction
2.7Å resolution

Crystal structure of Klebsiella pneumoniae RstA DNA-binding domain in complex with RstA box

Released:
DOI: 10.2210/pdb4nhj/pdb
PDB entry 4nhj coloured by chain, front view.
PDB entry 4nhj coloured by chain, side view.
PDB entry 4nhj coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Structural dynamics of the two-component response regulator RstA in recognition of promoter DNA element.
Li YC, Chang CK, Chang CF, Cheng YH, Fang PJ, Yu T, Chen SC, Li YC, Hsiao CD, Huang TH
Nucleic Acids Res 42 8777-88 (2014)
PMID: 24990372

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108043 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Response regulator (Activator) in two-component regulatory system with RstB (OmpR family) Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 13.5 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6T8N1 (Residues: 131-239; Coverage: 46%)
Gene names: KPN_01521, rstA
Sequence domains: Transcriptional regulatory protein, C terminal
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*GP*GP*TP*TP*GP*TP*AP*CP*AP*TP*TP*CP*CP*GP*TP*TP*AP*CP*TP*CP*CP*CP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 6.97 KDa
Source organism: Klebsiella pneumoniae
Expression system: Not provided
5'-D(*CP*AP*GP*GP*GP*AP*GP*TP*AP*AP*CP*GP*GP*AP*AP*TP*GP*TP*AP*CP*AP*AP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 7.15 KDa
Source organism: Klebsiella pneumoniae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21212
Unit cell:
a: 159.935Å b: 33.313Å c: 72.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.216 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

15 citation in other articles

Structure and dynamics of polymyxin-resistance-associated response regulator PmrA in complex with promoter DNA.
Lou et al. (2015)
14 more

4 mentions without citation

The Structure of the Biofilm-controlling Response Regulator BfmR from Acinetobacter baumannii Reveals Details of Its DNA-binding Mechanism.
Draughn et al. (2018)
3 more