› 4ni6

X-ray diffraction
1.1Å resolution

Repeat domain 1 of Clostridium perfringens CPE0147

Released:
DOI: 10.2210/pdb4ni6/pdb
PDB entry 4ni6 coloured by chain, front view.
PDB entry 4ni6 coloured by chain, side view.
PDB entry 4ni6 coloured by chain, top view.
Source organism: Clostridium perfringens B str. ATCC 3626
Primary publication:
Autocatalytically generated Thr-Gln ester bond cross-links stabilize the repetitive Ig-domain shaft of a bacterial cell surface adhesin.
Kwon H, Squire CJ, Young PG, Baker EN
Proc Natl Acad Sci U S A 111 1367-72 (2014)
PMID: 24344302

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative surface anchored protein Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.82 KDa
Source organism: Clostridium perfringens B str. ATCC 3626
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1R775 (Residues: 298-438; Coverage: 7%)
Gene name: AC1_0147
Sequence domains: T-Q ester bond containing domain
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 48.864Å b: 28.978Å c: 49.313Å
α: 90° β: 115.6° γ: 90°
R-values:
R R work R free
0.184 0.182 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Engineering of Metabolic Pathways by Artificial Enzyme Channels.
Pröschel et al. (2015)
5 more

1 mention without citation

Autocatalytically generated Thr-Gln ester bond cross-links stabilize the repetitive Ig-domain shaft of a bacterial cell surface adhesin.
Kwon et al. (2014)