› 4nmw

X-ray diffraction
1.5Å resolution

Crystal Structure of Carboxylesterase BioH from Salmonella enterica

Released:
DOI: 10.2210/pdb4nmw/pdb
PDB entry 4nmw coloured by chain, front view.
PDB entry 4nmw coloured by chain, side view.
PDB entry 4nmw coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Entry authors: Kim Y, Zhou M, Grimshaw S, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Pimeloyl-[acyl-carrier protein] methyl ester + H(2)O = pimeloyl-[acyl-carrier protein] + methanol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pimeloyl-[acyl-carrier protein] methyl ester esterase Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 28.94 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZLI9 (Residues: 1-256; Coverage: 100%)
Gene names: STM3509, bioH
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 99.059Å b: 54.211Å c: 66.769Å
α: 90° β: 126.13° γ: 90°
R-values:
R R work R free
0.143 0.141 0.179
Expression system: Escherichia coli BL21(DE3)

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