› 4nmy

X-ray diffraction
1.9Å resolution

Crystal Structure of the Thiamin-bound form of Substrate-binding Protein of ABC Transporter from Clostridium difficile

Released:
DOI: 10.2210/pdb4nmy/pdb
PDB entry 4nmy coloured by chain, front view.
PDB entry 4nmy coloured by chain, side view.
PDB entry 4nmy coloured by chain, top view.
Source organism: Clostridioides difficile 630
Entry authors: Kim Y, Zhou M, Grimshaw S, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172711 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SsuA/THI5-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 34.36 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q187U0 (Residues: 38-337; Coverage: 94%)
Gene name: CD630_19790
Sequence domains: NMT1/THI5 like
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand VIB 2 x VIB
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 49.529Å b: 76.867Å c: 154.901Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.203
Expression system: Escherichia coli BL21(DE3)

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