› 4nn5

X-ray diffraction
1.9Å resolution

Cytokine receptor complex - Crystal form 1A

Released:
DOI: 10.2210/pdb4nn5/pdb
PDB entry 4nn5 coloured by chain, front view.
PDB entry 4nn5 coloured by chain, side view.
PDB entry 4nn5 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of the proinflammatory signaling complex mediated by TSLP.
Verstraete K, van Schie L, Vyncke L, Bloch Y, Tavernier J, Pauwels E, Peelman F, Savvides SN
Nat Struct Mol Biol 21 375-82 (2014)
PMID: 24632570

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-147829 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thymic stromal lymphopoietin Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 15.11 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9JIE6 (Residues: 20-140; Coverage: 100%)
Gene name: Tslp
Sequence domains: Thymic stromal lymphopoietin
Structure domains: Thymic stromal lymphopoietin
Interleukin-7 receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 223 amino acids
Theoretical weight: 25.43 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16872 (Residues: 21-239; Coverage: 50%)
Gene name: Il7r
Sequence domains:
Structure domains:
Cytokine receptor-like factor 2 Chain: C
Molecule details ›
Chain: C
Length: 212 amino acids
Theoretical weight: 22.81 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8CII9 (Residues: 20-222; Coverage: 60%)
Gene names: Crlf2, Crlm2, Tpte2, Tslpr
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21212
Unit cell:
a: 150.06Å b: 79.756Å c: 52.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.201
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Tuning the Cytokine Responses: An Update on Interleukin (IL)-4 and IL-13 Receptor Complexes.
Junttila IS. (2018)
9 more

2 mentions without citation

Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma.
Verstraete et al. (2017)
1 more