› 4noi

X-ray diffraction
2.17Å resolution

2.17 Angstrom Crystal Structure of DNA-directed RNA Polymerase Subunit Alpha from Campylobacter jejuni.

Released:
DOI: 10.2210/pdb4noi/pdb
Model geometry
Fit model/data
PDB entry 4noi coloured by chain, front view.
PDB entry 4noi coloured by chain, side view.
PDB entry 4noi coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Entry authors: Minasov G, Shuvalova L, Dubrovska I, Winsor J, Grimshaw S, Kwon K, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 361 amino acids
Theoretical weight: 40.8 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9PM80 (Residues: 1-337; Coverage: 100%)
Gene names: Cj1595, rpoA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand IOD 4 x IOD
Ligand CL 12 x CL
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 99.173Å b: 62.743Å c: 93.621Å
α: 90° β: 112.52° γ: 90°
R-values:
R R work R free
0.176 0.174 0.225
Expression system: Escherichia coli

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