› 4nol

X-ray diffraction
2.7Å resolution

Crystal structure of proenzyme asparaginyl endopeptidase (AEP)/Legumain mutant D233A at pH 7.5

Released:
DOI: 10.2210/pdb4nol/pdb
Model geometry
Fit model/data
PDB entry 4nol coloured by chain, front view.
PDB entry 4nol coloured by chain, side view.
PDB entry 4nol coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stage.
Zhao L, Hua T, Crowley C, Ru H, Ni X, Shaw N, Jiao L, Ding W, Qu L, Hung LW, Huang W, Liu L, Ye K, Ouyang S, Cheng G, Liu ZJ
Cell Res 24 344-58 (2014)
PMID: 24407422

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Legumain Chains: A, B
Molecule details ›
Chains: A, B
Length: 441 amino acids
Theoretical weight: 50.22 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O89017 (Residues: 1-435; Coverage: 100%)
Gene names: Lgmn, Prsc1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 51.962Å b: 163.361Å c: 59.278Å
α: 90° β: 104.32° γ: 90°
R-values:
R R work R free
0.275 0.217 0.275
Expression system: Spodoptera frugiperda

Quick links

Citations

17 review citations

The endosomal-lysosomal system: from acidification and cargo sorting to neurodegeneration.
Hu et al. (2015)
16 more

2 mentions without citation

Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stage.
Zhao et al. (2014)
1 more