› 4nq3

X-ray diffraction
2.7Å resolution

Crystal structure of cyanuic acid hydrolase from A. caulinodans

Released:
DOI: 10.2210/pdb4nq3/pdb
PDB entry 4nq3 coloured by chain, front view.
PDB entry 4nq3 coloured by chain, side view.
PDB entry 4nq3 coloured by chain, top view.
Source organism: Azorhizobium caulinodans ORS 571
Primary publication:
Cyanuric acid hydrolase from Azorhizobium caulinodans ORS 571: crystal structure and insights into a new class of Ser-Lys dyad proteins.
Cho S, Shi K, Seffernick JL, Dodge AG, Wackett LP, Aihara H
PLoS One 9 e99349 (2014)
PMID: 24915109

Function and Biology Details

Reaction catalysed: 
Cyanuric acid + H(2)O = 1-carboxybiuret
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyanuric acid amidohydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 356 amino acids
Theoretical weight: 36.74 KDa
Source organism: Azorhizobium caulinodans ORS 571
Expression system: Escherichia coli
UniProt:
  • Canonical: A8IKD2 (Residues: 1-356; Coverage: 100%)
Gene name: AZC_3892
Sequence domains: Amidohydrolase ring-opening protein (Amido_AtzD_TrzD)
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand BR8 3 x BR8
Ligand SO4 10 x SO4
Ligand MG 3 x MG
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: I4122
Unit cell:
a: 237.866Å b: 237.866Å c: 105.313Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.191
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Bacterial Cyanuric Acid Hydrolase for Water Treatment.
Yeom et al. (2015)
4 more

4 mentions without citation

Ancient Evolution and Recent Evolution Converge for the Biodegradation of Cyanuric Acid and Related Triazines.
Seffernick et al. (2016)
3 more