› 4nq8

X-ray diffraction
1.5Å resolution

Crystal structure of a trap periplasmic solute binding protein from Bordetella bronchispeptica (bb3421), target EFI-510039, with density modeled as pantoate

Released:
DOI: 10.2210/pdb4nq8/pdb
Model geometry
Fit model/data
PDB entry 4nq8 coloured by chain, front view.
PDB entry 4nq8 coloured by chain, side view.
PDB entry 4nq8 coloured by chain, top view.
Source organism: Bordetella bronchiseptica RB50
Primary publication:
Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting MW, Al-Obaidi N, Zhao S, San Francisco B, Kim J, Wichelecki DJ, Bouvier JT, Solbiati JO, Vu H, Zhang X, Rodionov DA, Love JD, Hillerich BS, Seidel RD, Quinn RJ, Osterman AL, Cronan JE, Jacobson MP, Gerlt JA, Almo SC
OpenAccess logo Biochemistry 54 909-31 (2015)
PMID: 25540822

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-102089 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic substrate-binding transport protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 37.03 KDa
Source organism: Bordetella bronchiseptica RB50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3LPK2 (Residues: 1-324; Coverage: 100%)
Gene name: BB3421
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

3 bound ligands:
Ligand PAF 2 x PAF
Ligand SO4 2 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P1
Unit cell:
a: 39.004Å b: 56.352Å c: 75.609Å
α: 99.21° β: 92.01° γ: 106.23°
R-values:
R R work R free
0.159 0.158 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
12 more

1 mention without citation

Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting et al. (2015)