› 4ntt

X-ray diffraction
3.5Å resolution

Structure of the catalytic subunit of cAMP-dependent protein kinase bound to ADP and one magnesium ion

Released:
DOI: 10.2210/pdb4ntt/pdb
PDB entry 4ntt coloured by chain, front view.
PDB entry 4ntt coloured by chain, side view.
PDB entry 4ntt coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Molecular features of product release for the PKA catalytic cycle.
Bastidas AC, Wu J, Taylor SS
Biochemistry 54 2-10 (2015)
PMID: 25077557

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase catalytic subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 40.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 2-7, 9-351, 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
2 modified residues:
Ligand TPO 2 x TPO
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 50.48Å b: 143.15Å c: 62.57Å
α: 90° β: 103.61° γ: 90°
R-values:
R R work R free
0.23 0.227 0.284
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Evolution of a dynamic molecular switch.
Taylor et al. (2019)
2 more

6 mentions without citation

Globally correlated conformational entropy underlies positive and negative cooperativity in a kinase's enzymatic cycle.
Wang et al. (2019)
5 more