› 4nu6

X-ray diffraction
2.65Å resolution

Crystal Structure of PTDH R301K

Released:
DOI: 10.2210/pdb4nu6/pdb
PDB entry 4nu6 coloured by chain, front view.
PDB entry 4nu6 coloured by chain, side view.
PDB entry 4nu6 coloured by chain, top view.
Source organism: Pseudomonas stutzeri
Primary publication:
Chemical rescue and inhibition studies to determine the role of Arg301 in phosphite dehydrogenase.
Hung JE, Fogle EJ, Garg N, Chekan JR, Nair SK, van der Donk WA
PLoS One 9 e87134 (2014)
PMID: 24498026

Function and Biology Details

Reaction catalysed: 
Phosphonate + NAD(+) + H(2)O = phosphate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphonate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 35.88 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli
UniProt:
  • Canonical: O69054 (Residues: 1-329; Coverage: 98%)
Gene name: ptxD
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21212
Unit cell:
a: 116.119Å b: 81.472Å c: 82.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.255
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

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Johnson et al. (2020)
6 more

2 mentions without citation

Structural, Biochemical, and Evolutionary Characterizations of Glyoxylate/Hydroxypyruvate Reductases Show Their Division into Two Distinct Subfamilies.
Kutner et al. (2018)
1 more