› 4nuh

X-ray diffraction
1.34Å resolution

Crystal structure of mLeIBP, a capping head region swapped mutant of ice-binding protein

Released:
DOI: 10.2210/pdb4nuh/pdb
PDB entry 4nuh coloured by chain, front view.
PDB entry 4nuh coloured by chain, side view.
PDB entry 4nuh coloured by chain, top view.
Source organism: Leucosporidium
Primary publication:
Structure-based characterization and antifreeze properties of a hyperactive ice-binding protein from the Antarctic bacterium Flavobacterium frigoris PS1.
Do H, Kim SJ, Kim HJ, Lee JH
Acta Crystallogr D Biol Crystallogr 70 1061-73 (2014)
PMID: 24699650

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124887 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ice-binding protein Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 21.92 KDa
Source organism: Leucosporidium
Expression system: Escherichia coli
UniProt:
  • Canonical: H7FWB6 (Residues: 63-64, 65-275; Coverage: 85%)
Gene name: HJ01_03463
Sequence domains: Ice-binding-like

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P1
Unit cell:
a: 29.358Å b: 31.222Å c: 47.051Å
α: 76.32° β: 72.59° γ: 76.62°
R-values:
R R work R free
0.181 0.163 0.201
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant.
Kim et al. (2017)
6 more

1 mention without citation

Large-Scale Discovery of Microbial Fibrillar Adhesins and Identification of Novel Members of Adhesive Domain Families.
Monzon et al. (2022)