› 4nxk

X-ray diffraction
2.3Å resolution

Crystal structure of Abp-D197A, a catalytic mutant of a GH27-b-L-arabinopyranosidase from Geobacillus stearothermophilus

Released:
DOI: 10.2210/pdb4nxk/pdb
PDB entry 4nxk coloured by chain, front view.
PDB entry 4nxk coloured by chain, side view.
PDB entry 4nxk coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structure-specificity relationships in Abp, a GH27 β-L-arabinopyranosidase from Geobacillus stearothermophilus T6.
Lansky S, Salama R, Solomon HV, Feinberg H, Belrhali H, Shoham Y, Shoham G
Acta Crystallogr D Biol Crystallogr 70 2994-3012 (2014)
PMID: 25372689

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164923 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Abp, a GH27 beta-L-arabinopyranosidase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 448 amino acids
Theoretical weight: 51.2 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 66 x SO4
Ligand GOL 30 x GOL
Ligand CIT 6 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 107.701Å b: 203.525Å c: 286.946Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.201
Expression system: Escherichia coli

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Citations

1 review citation

The Role of Arabinogalactan Type II Degradation in Plant-Microbe Interactions.
Villa-Rivera et al. (2021)
4 other articles