› 4nz7

X-ray diffraction
2.7Å resolution

Steroid receptor RNA Activator (SRA) modification by the human Pseudouridine Synthase 1 (hPus1p): RNA binding, activity, and atomic model

Released:
DOI: 10.2210/pdb4nz7/pdb
PDB entry 4nz7 coloured by chain, front view.
PDB entry 4nz7 coloured by chain, side view.
PDB entry 4nz7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Steroid receptor RNA activator (SRA) modification by the human pseudouridine synthase 1 (hPus1p): RNA binding, activity, and atomic model.
Huet T, Miannay FA, Patton JR, Thore S
PLoS One 9 e94610 (2014)
PMID: 24722331

Function and Biology Details

Reaction catalysed: 
tRNA uridine(38-40) = tRNA pseudouridine(38-40)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195323 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pseudouridylate synthase 1 homolog Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 35.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y606 (Residues: 83-394; Coverage: 73%)
Gene names: PP8985, PUS1
Sequence domains: tRNA pseudouridine synthase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EPE 1 x EPE
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Unit cell:
a: 39.446Å b: 69.044Å c: 116.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.183 0.238
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Pseudouridine: still mysterious, but never a fake (uridine)!
Spenkuch et al. (2014)
3 more

1 mention without citation

Steroid receptor RNA activator (SRA) modification by the human pseudouridine synthase 1 (hPus1p): RNA binding, activity, and atomic model.
Huet et al. (2014)