› 4nzg

X-ray diffraction
2.15Å resolution

Crystal Structure of the N-terminal domain of Moloney murine leukemia virus integrase, Northeast Structural Genomics Consortium Target OR3

Released:
DOI: 10.2210/pdb4nzg/pdb
PDB entry 4nzg coloured by chain, front view.
PDB entry 4nzg coloured by chain, side view.
PDB entry 4nzg coloured by chain, top view.
Source organism: Moloney murine leukemia virus isolate Shinnick
Primary publication:
X-ray crystal structure of the N-terminal region of Moloney murine leukemia virus integrase and its implications for viral DNA recognition.
Guan R, Aiyer S, Cote ML, Xiao R, Jiang M, Acton TB, Roth MJ, Montelione GT
Proteins 85 647-656 (2017)
PMID: 28066922

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 100 amino acids
Theoretical weight: 11.75 KDa
Source organism: Moloney murine leukemia virus isolate Shinnick
Expression system: Escherichia coli
UniProt:
  • Canonical: P03355 (Residues: 1338-1435; Coverage: 6%)
Gene name: gag-pol
Sequence domains: Zinc-finger like, probable DNA-binding
Structure domains: Endonuclease III; domain 1

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand ACT 3 x ACT
Ligand DTT 9 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 44.659Å b: 38.44Å c: 135.275Å
α: 90° β: 91.7° γ: 90°
R-values:
R R work R free
0.236 0.234 0.265
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and function of retroviral integrase.
Maertens et al. (2022)
1 more

1 mention without citation

X-ray crystal structure of the N-terminal region of Moloney murine leukemia virus integrase and its implications for viral DNA recognition.
Guan et al. (2017)