› 4nzi

X-ray diffraction
2.1Å resolution

Crystal structure of murine neuroglobin mutant V140W

Released:
DOI: 10.2210/pdb4nzi/pdb
PDB entry 4nzi coloured by chain, front view.
PDB entry 4nzi coloured by chain, side view.
PDB entry 4nzi coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Engineering the internal cavity of neuroglobin demonstrates the role of the haem-sliding mechanism.
Avella G, Ardiccioni C, Scaglione A, Moschetti T, Rondinelli C, Montemiglio LC, Savino C, Giuffrè A, Brunori M, Vallone B
Acta Crystallogr D Biol Crystallogr 70 1640-8 (2014)
PMID: 24914975

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190170 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuroglobin Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.39 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ER97 (Residues: 1-151; Coverage: 100%)
Gene name: Ngb
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: R32
Unit cell:
a: 87.214Å b: 87.214Å c: 114.399Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.219 0.262
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Neuroglobin: From structure to function in health and disease.
Ascenzi et al. (2016)
3 more

1 mention without citation

Mapping Hydrophobic Tunnels and Cavities in Neuroglobin with Noble Gas under Pressure.
Colloc'h et al. (2017)