› 4o0l

X-ray diffraction
2.2Å resolution

Crystal structure of NADPH-Dependent 3-Quinuclidinone Reductase from Rhodotorula Rubra

Released:
DOI: 10.2210/pdb4o0l/pdb
PDB entry 4o0l coloured by chain, front view.
PDB entry 4o0l coloured by chain, side view.
PDB entry 4o0l coloured by chain, top view.
Source organism: Rhodotorula mucilaginosa
Primary publication:
Structural basis of stereospecific reduction by quinuclidinone reductase.
Takeshita D, Kataoka M, Miyakawa T, Miyazono K, Kumashiro S, Nagai T, Urano N, Uzura A, Nagata K, Shimizu S, Tanokura M
AMB Express 4 6 (2014)
PMID: 24507746

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH-dependent 3-quinuclidinone reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 275 amino acids
Theoretical weight: 29.38 KDa
Source organism: Rhodotorula mucilaginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: B9ZZZ6 (Residues: 1-272; Coverage: 100%)
Gene name: rqr
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NDP 4 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P41212
Unit cell:
a: 91.272Å b: 91.272Å c: 265.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.223
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Enzymes useful for chiral compound synthesis: structural biology, directed evolution, and protein engineering for industrial use.
Kataoka et al. (2016)
1 more

2 mentions without citation

Structural and biochemical characterization of 20β-hydroxysteroid dehydrogenase from Bifidobacterium adolescentis strain L2-32.
Doden et al. (2019)
1 more