› 4o0q

X-ray diffraction
1.92Å resolution

Apo structure of a methyltransferase component involved in O-demethylation

Released:
DOI: 10.2210/pdb4o0q/pdb
PDB entry 4o0q coloured by chain, front view.
PDB entry 4o0q coloured by chain, side view.
PDB entry 4o0q coloured by chain, top view.
Source organism: Desulfitobacterium hafniense DCB-2
Primary publication:
Structures of the methyltransferase component of Desulfitobacterium hafniense DCB-2 O-demethylase shed light on methyltetrahydrofolate formation.
Sjuts H, Dunstan MS, Fisher K, Leys D
Acta Crystallogr D Biol Crystallogr 71 1900-8 (2015)
PMID: 26327380

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pterin-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 31.76 KDa
Source organism: Desulfitobacterium hafniense DCB-2
Expression system: Escherichia coli
UniProt:
  • Canonical: B8FW00 (Residues: 2-269; Coverage: 100%)
Gene name: Dhaf_0722
Sequence domains: Pterin binding enzyme
Structure domains: Dihydropteroate synthase-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21212
Unit cell:
a: 90.67Å b: 119.07Å c: 58.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.227
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

An Ecological Basis for Dual Genetic Code Expansion in Marine Deltaproteobacteria.
Kivenson et al. (2021)
2 more