4o26

X-ray diffraction
3Å resolution

Crystal structure of the TRBD domain of TERT and the CR4/5 of TR

Released:
Source organism: Oryzias latipes
Primary publication:
Structural basis for protein-RNA recognition in telomerase.
Nat Struct Mol Biol 21 507-12 (2014)
PMID: 24793650

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-172989 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Telomerase reverse transcriptase Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.99 KDa
Source organism: Oryzias latipes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1PS67 (Residues: 318-572; Coverage: 23%)
Gene name: tert
Sequence domains: Telomerase ribonucleoprotein complex - RNA binding domain
Structure domains: Topoisomerase I; Chain A, domain 4
Telomerase TR Chains: E, F
Molecule details ›
Chains: E, F
Length: 51 nucleotides
Theoretical weight: 16.36 KDa
Source organism: Oryzias latipes
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P6222
Unit cell:
a: 118.092Å b: 118.092Å c: 358.021Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.261
Expression systems:
  • Escherichia coli
  • Not provided