› 4o2l

X-ray diffraction
2.4Å resolution

Structure of Mus musculus Rheb G63A mutant bound to GTP

Released:
DOI: 10.2210/pdb4o2l/pdb
PDB entry 4o2l coloured by chain, front view.
PDB entry 4o2l coloured by chain, side view.
PDB entry 4o2l coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure-guided mutation of the conserved G3-box glycine in Rheb generates a constitutively activated regulator of mammalian target of rapamycin (mTOR).
Mazhab-Jafari MT, Marshall CB, Ho J, Ishiyama N, Stambolic V, Ikura M
J Biol Chem 289 12195-201 (2014)
PMID: 24648513

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187642 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP-binding protein Rheb Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 19.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q921J2 (Residues: 1-169; Coverage: 92%)
Gene name: Rheb
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand TRS 1 x TRS
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Unit cell:
a: 57.557Å b: 70.248Å c: 79.733Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.243
Expression system: Escherichia coli

Quick links

Citations

3 review citations

PIKKs--the solenoid nest where partners and kinases meet.
Baretić et al. (2014)
2 more

1 mention without citation

Structure-guided mutation of the conserved G3-box glycine in Rheb generates a constitutively activated regulator of mammalian target of rapamycin (mTOR).
Mazhab-Jafari et al. (2014)