› 4o3x

X-ray diffraction
2.5Å resolution

Crystal structure of the vaccine antigen Transferrin Binding Protein B (TbpB) mutant Phe-171-Ala from Actinobacillus pleuropneumoniae H49

Released:
DOI: 10.2210/pdb4o3x/pdb
PDB entry 4o3x coloured by chain, front view.
PDB entry 4o3x coloured by chain, side view.
PDB entry 4o3x coloured by chain, top view.
Source organism: Actinobacillus pleuropneumoniae
Primary publication:
Nonbinding site-directed mutants of transferrin binding protein B exhibit enhanced immunogenicity and protective capabilities.
Frandoloso R, Martínez-Martínez S, Calmettes C, Fegan J, Costa E, Curran D, Yu RH, Gutiérrez-Martín CB, Rodríguez-Ferri EF, Moraes TF, Schryvers AB
Infect Immun 83 1030-8 (2015)
PMID: 25547790

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124129 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transferrin-binding protein B Chain: B
Molecule details ›
Chain: B
Length: 255 amino acids
Theoretical weight: 28.03 KDa
Source organism: Actinobacillus pleuropneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0T4F6 (Residues: 56-308; Coverage: 46%)
Gene name: tbpB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P61
Unit cell:
a: 41.38Å b: 41.38Å c: 234Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.195 0.248
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Outer membrane vesicles as platform vaccine technology.
van der Pol et al. (2015)
10 more

1 mention without citation

Nonbinding site-directed mutants of transferrin binding protein B exhibit enhanced immunogenicity and protective capabilities.
Frandoloso et al. (2015)