› 4o4u

X-ray diffraction
2.64Å resolution

Crystal structure of the vaccine antigen Transferrin Binding Protein B (TbpB) mutant Trp-176-Ala from Haemophilus parasuis Hp5

Released:
DOI: 10.2210/pdb4o4u/pdb
PDB entry 4o4u coloured by chain, front view.
PDB entry 4o4u coloured by chain, side view.
PDB entry 4o4u coloured by chain, top view.
Source organism: Glaesserella parasuis
Primary publication:
Nonbinding site-directed mutants of transferrin binding protein B exhibit enhanced immunogenicity and protective capabilities.
Frandoloso R, Martínez-Martínez S, Calmettes C, Fegan J, Costa E, Curran D, Yu RH, Gutiérrez-Martín CB, Rodríguez-Ferri EF, Moraes TF, Schryvers AB
Infect Immun 83 1030-8 (2015)
PMID: 25547790

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TbpB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 532 amino acids
Theoretical weight: 58.39 KDa
Source organism: Glaesserella parasuis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2UKY5 (Residues: 1-532; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 81.03Å b: 135.7Å c: 103.96Å
α: 90° β: 100.05° γ: 90°
R-values:
R R work R free
0.216 0.214 0.266
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Outer membrane vesicles as platform vaccine technology.
van der Pol et al. (2015)
10 more

3 mentions without citation

Nonbinding site-directed mutants of transferrin binding protein B exhibit enhanced immunogenicity and protective capabilities.
Frandoloso et al. (2015)
2 more