› 4o5p

X-ray diffraction
2Å resolution

Crystal structure of an uncharacterized protein from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4o5p/pdb
PDB entry 4o5p coloured by chain, front view.
PDB entry 4o5p coloured by chain, side view.
PDB entry 4o5p coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure of the type VI secretion phospholipase effector Tle1 provides insight into its hydrolysis and membrane targeting.
Hu H, Zhang H, Gao Z, Wang D, Liu G, Xu J, Lan K, Dong Y
Acta Crystallogr D Biol Crystallogr 70 2175-85 (2014)
PMID: 25084336

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191170 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tle1 phospholipase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 884 amino acids
Theoretical weight: 97.57 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HYV3 (Residues: 1-882; Coverage: 100%)
Gene name: PA3290
Sequence domains: Uncharacterized alpha/beta hydrolase domain (DUF2235)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P1
Unit cell:
a: 61.603Å b: 81.078Å c: 92.145Å
α: 97.83° β: 89.96° γ: 90.01°
R-values:
R R work R free
0.228 0.226 0.269
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The T6SSs of Pseudomonas aeruginosa Strain PAO1 and Their Effectors: Beyond Bacterial-Cell Targeting.
Sana et al. (2016)
3 more

2 mentions without citation

Bacterial Sphingomyelinases and Phospholipases as Virulence Factors.
Flores-Díaz et al. (2016)
1 more