› 4o66

X-ray diffraction
1.9Å resolution

Crystal Structure of SMARCAL1 HARP substrate recognition domain

Released:
DOI: 10.2210/pdb4o66/pdb
PDB entry 4o66 coloured by chain, front view.
PDB entry 4o66 coloured by chain, side view.
PDB entry 4o66 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A structure-specific nucleic acid-binding domain conserved among DNA repair proteins.
Mason AC, Rambo RP, Greer B, Pritchett M, Tainer JA, Cortez D, Eichman BF
Proc Natl Acad Sci U S A 111 7618-23 (2014)
PMID: 24821763

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183880 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 76 amino acids
Theoretical weight: 8.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8BJL0 (Residues: 197-268; Coverage: 8%)
Gene names: Harp, Smarcal1
Sequence domains: HepA-related protein (HARP)

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 56.511Å b: 56.634Å c: 104.321Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.168 0.197
Expression system: Escherichia coli

Quick links

Citations

9 review citations

RPA and RAD51: fork reversal, fork protection, and genome stability.
Bhat et al. (2018)
8 more

2 mentions without citation

A structure-specific nucleic acid-binding domain conserved among DNA repair proteins.
Mason et al. (2014)
1 more