› 4o6m

X-ray diffraction
1.9Å resolution

Structure of AF2299, a CDP-alcohol phosphotransferase (CMP-bound)

Released:
DOI: 10.2210/pdb4o6m/pdb
PDB entry 4o6m coloured by chain, front view.
PDB entry 4o6m coloured by chain, side view.
PDB entry 4o6m coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Structural basis for catalysis in a CDP-alcohol phosphotransferase.
Sciara G, Clarke OB, Tomasek D, Kloss B, Tabuso S, Byfield R, Cohn R, Banerjee S, Rajashankar KR, Slavkovic V, Graziano JH, Shapiro L, Mancia F
Nat Commun 5 4068 (2014)
PMID: 24923293

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127926 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CDP-alcohol phosphatidyltransferase AF-2299-like N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 372 amino acids
Theoretical weight: 42.26 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O27985 (Residues: 1-344; Coverage: 100%)
Gene name: AF_2299
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand MPG 24 x MPG
Ligand SO4 8 x SO4
Ligand C5P 1 x C5P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 46.363Å b: 91.148Å c: 107.023Å
α: 90° β: 92.05° γ: 90°
R-values:
R R work R free
0.2 0.198 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

From yeast to humans - roles of the Kennedy pathway for phosphatidylcholine synthesis.
McMaster CR. (2018)
3 more

9 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
8 more