› 4o7v

X-ray diffraction
2.3Å resolution

SAICAR synthetase (Type-2) in complex with ADP and UDP/UMP

Released:
DOI: 10.2210/pdb4o7v/pdb
PDB entry 4o7v coloured by chain, front view.
PDB entry 4o7v coloured by chain, side view.
PDB entry 4o7v coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Manjunath K, Jeyakanthan J, Sekar K

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129650 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 27.48 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57978 (Residues: 1-238; Coverage: 100%)
Gene names: PH0239, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand UDP 1 x UDP
Ligand ADP 1 x ADP
Ligand U5P 1 x U5P
Ligand ACT 1 x ACT
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2221
Unit cell:
a: 44.16Å b: 155.12Å c: 78.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.249
Expression system: Escherichia coli

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