› 4oa8

X-ray diffraction
2.15Å resolution

X-ray crystal structure of O-methyltransferase from Anaplasma phagocytophilum in apo form

Released:
DOI: 10.2210/pdb4oa8/pdb
PDB entry 4oa8 coloured by chain, front view.
PDB entry 4oa8 coloured by chain, side view.
PDB entry 4oa8 coloured by chain, top view.
Source organism: Anaplasma phagocytophilum str. HZ
Primary publication:
An O-Methyltransferase Is Required for Infection of Tick Cells by Anaplasma phagocytophilum.
Oliva Chávez AS, Fairman JW, Felsheim RF, Nelson CM, Herron MJ, Higgins L, Burkhardt NY, Oliver JD, Markowski TW, Kurtti TJ, Edwards TE, Munderloh UG
PLoS Pathog 11 e1005248 (2015)
PMID: 26544981

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 25.31 KDa
Source organism: Anaplasma phagocytophilum str. HZ
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2GKC7 (Residues: 2-218; Coverage: 100%)
Gene name: APH_0584
Sequence domains: O-methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Unit cell:
a: 73.15Å b: 76.4Å c: 79.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.232
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Microbial Invasion vs. Tick Immune Regulation.
Sonenshine et al. (2017)
2 more

1 mention without citation

An O-Methyltransferase Is Required for Infection of Tick Cells by Anaplasma phagocytophilum.
Oliva Chávez et al. (2015)