› 4oaa

X-ray diffraction
3.5Å resolution

Crystal structure of E. coli lactose permease G46W,G262W bound to sugar

Released:
DOI: 10.2210/pdb4oaa/pdb
PDB entry 4oaa coloured by chain, front view.
PDB entry 4oaa coloured by chain, side view.
PDB entry 4oaa coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. DH10B
Primary publication:
Structure of sugar-bound LacY.
Kumar H, Kasho V, Smirnova I, Finer-Moore JS, Kaback HR, Stroud RM
Proc Natl Acad Sci U S A 111 1784-8 (2014)
PMID: 24453216

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164942 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lactose/galactose transporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 46.79 KDa
Source organism: Escherichia coli str. K-12 substr. DH10B
Expression system: Escherichia coli
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

Carbohydrate polymer : NEW Components: YIO, GAL
Ligand YIO 2 x YIO
Ligand GAL 2 x GAL
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 101.56Å b: 121.94Å c: 264.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.256 0.293
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Insights into Protein-Ligand Interactions: Mechanisms, Models, and Methods.
Du et al. (2016)
14 more

24 mentions without citation

Understanding transporter specificity and the discrete appearance of channel-like gating domains in transporters.
Diallinas G. (2014)
23 more