› 4oap

X-ray diffraction
1.93Å resolution

An Axe2 mutant (W190I), an acetyl-xylooligosaccharide esterase from Geobacillus Stearmophilus

Released:
DOI: 10.2210/pdb4oap/pdb
PDB entry 4oap coloured by chain, front view.
PDB entry 4oap coloured by chain, side view.
PDB entry 4oap coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Entry authors: Lansky S, Alalouf O, Solomon HV, Belrhali H, Shoham Y, Shoham G

Function and Biology Details

Reaction catalysed: 
Deacetylation of xylans and xylo-oligosaccharides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylxylan esterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.73 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09LX1 (Residues: 1-219; Coverage: 100%)
Gene name: axe2
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: SGNH hydrolase

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand GOL 10 x GOL
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I422
Unit cell:
a: 109.95Å b: 109.95Å c: 213.342Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.18
Expression system: Escherichia coli

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