› 4ocq

X-ray diffraction
1.88Å resolution

N-acetylhexosamine 1-phosphate kinase in complex with GalNAc

Released:
DOI: 10.2210/pdb4ocq/pdb
PDB entry 4ocq coloured by chain, front view.
PDB entry 4ocq coloured by chain, side view.
PDB entry 4ocq coloured by chain, top view.
Source organism: Bifidobacterium longum
Primary publication:
Insights into the binding specificity and catalytic mechanism of N-acetylhexosamine 1-phosphate kinases through multiple reaction complexes.
Wang KC, Lyu SY, Liu YC, Chang CY, Wu CJ, Li TL
Acta Crystallogr D Biol Crystallogr 70 1401-10 (2014)
PMID: 24816108

Function and Biology Details

Reaction catalysed: 
ATP + N-acetyl-D-hexosamine = ADP + N-acetyl-alpha-D-hexosamine 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylhexosamine 1-kinase Chain: A
Molecule details ›
Chain: A
Length: 379 amino acids
Theoretical weight: 42.1 KDa
Source organism: Bifidobacterium longum
Expression system: Escherichia coli
UniProt:
  • Canonical: E8MF12 (Residues: 1-359; Coverage: 100%)
Gene names: BLLJ_1622, lnpB, nahK
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

1 bound ligand:
Ligand A2G 1 x A2G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P212121
Unit cell:
a: 55.212Å b: 79.623Å c: 98.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.218
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes.
Fushinobu et al. (2021)
3 other articles

1 mention without citation

Structural and kinetic characterization of (S)-1-amino-2-propanol kinase from the aminoacetone utilization microcompartment of Mycobacterium smegmatis.
Mallette et al. (2018)