› 4ocy

X-ray diffraction
2.79Å resolution

Fab for methotrexate (unbound apo)

Released:
DOI: 10.2210/pdb4ocy/pdb
PDB entry 4ocy coloured by chain, front view.
PDB entry 4ocy coloured by chain, side view.
PDB entry 4ocy coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Water channel in the binding site of a high affinity anti-methotrexate antibody.
Gayda S, Longenecker KL, Manoj S, Judge RA, Saldana SC, Ruan Q, Swift KM, Tetin SY
Biochemistry 53 3719-26 (2014)
PMID: 24832237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207565 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab ADD058 Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 216 amino acids
Theoretical weight: 23.24 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.78 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: A2NHM3 (Residues: 1-216; Coverage: 99%)
Gene names: Igk-C, Igkc
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P213
Unit cell:
a: 144.369Å b: 144.369Å c: 144.369Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.206
Expression system: Homo sapiens

Quick links

Citations

2 citation in other articles

Bioluminescent Antibodies for Point-of-Care Diagnostics.
Xue et al. (2017)
1 more

1 mention without citation

Structural Analysis of Anti-Hapten Antibodies to Identify Long-Range Structural Movements Induced by Hapten Binding.
Al Qaraghuli et al. (2021)