Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
CRISPR-associated endonuclease Cas9 (preferred)
PDBe Complex ID:
PDB-CPX-125297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas9
Molecule details ›
Chain: A
Length: 1101 amino acids
Theoretical weight: 124.9 KDa
Source organism: Actinomyces naeslundii str. Howell 279
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 1101 amino acids
Theoretical weight: 124.9 KDa
Source organism: Actinomyces naeslundii str. Howell 279
Expression system: Escherichia coli
UniProt:
- Canonical:
J3F2B0 (Residues: 1-1101; Coverage: 100%)
Sequence domains:
- Cas9 alpha-helical lobe domain
- HNH endonuclease
- RuvC endonuclease subdomain 3
- CRISPR-associated endonuclease Cas9 beta-hairpin domain
- Topo homolgy domain in CRISPR-associated endonuclease Cas9
- Cas9 C-terminal domain
Ligands and Environments
Experiments and Validation Details
wwPDB Validation report is not available for this entry.
X-ray source:
ALS BEAMLINE 8.3.1, ALS BEAMLINE 8.2.2
Spacegroup:
P21
Expression system: Escherichia coli
